The main goal of this study was the evaluation of physical properties of wheat-oat bread baked with yeast, with or without rye sourdough and with different share of residual oat flour (30% or 50% relative to the total weight of the flour). These breads were compared with identical breads made of commercial oat flour. The adopted quality indicators included the following: sensory assessment, bread volume, crumb moisture and crumb mechanical properties (met. TPA), both on baking day and during 3-day storage. The breads with 30% and 50% share of residual oat flour, regardless of the dough making methods, obtained a sensory evaluation in the first quality class, wherein the wheat–oat breads with rye sourdough obtained a better score for taste and smell. Breads with 30% and 50% share of residual oat flour were characterised by larger volume than those of commercial oat flour, irrespective of the dough making methods. Crumb of the breads with 30% and 50% share of residual oat flour was characterised by lower hardness both on baking day and after 72 hours of storage, for breads of commercial oat flour, regardless of the dough making method. Breads with rye sourdough were characterised by a lower increase of crumb hardness during storage than breads baked with yeast only, regardless of the quantity participation and type of oat flour used. Using rye sourdough influenced favourably the process of staling of wheat-oat breads, both with 30% or 50% share of the two types of oat flour, not only the crumb texture parameters, but also reduced the loss of water during storage.