Samples of rapeseed cakes with or without antioxidant were stored in a laboratory, with or without access of light and air, for a period of six months. Acid value and peroxide value were analysed at the beginning and every month in three replications for each series. On the day of research beginning and after 6 months of storage of the rapeseed cakes the content of basic nutrients and fatty acid profiles in lipid fraction were estimated. An increase of acid value of rapeseed cakes lipid fraction during whole storage time was proved. The highest acid values were obtained for the research material stored without light and air. A significant (P≤0.01) decrease of peroxide value was noted, irrespective of the conditions storage, after the addition of antioxidant. After 6 months of storage a decrease of crude fat and an increase of crude fibre in the rapeseed cakes were found. An increase of saturated fatty acids (SFA) and a decrease of unsaturated (UFA), including polyunsaturated fatty acids (PUFA), were found in the lipid fraction.