The effect of NaCl content in Hoagland’s medium at concentration of 50, 100, 150 mmol dm^-3 on growth and development of barley cv. Promyk and Suweren was studied under controlled conditions. After 28 days of growth, the gas exchange and biometric parameters were measured. Results showed that increasing concentrations of NaCl in the culture medium lead to significant inhibition of growth of leaves and of the average value of fresh and dry matter of the aboveground part and roots. It was found that the intensity of CO₂ assimilation decreased with increasing NaCl concentration in medium culture. Stimulating effect of NaCl on roots length was observed at a low concentration of 50 mmol dm^-3.